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Overview of the order form

The order form consists of two sheets:
  1. The Order information sheet requests general information about your order
  2. The Sequence order sheet is where you can input the sequences you want to test and gain data on. Image of both sheets of the order form

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Step-by-step guide on how to fill out the order form

1

Fill out order information sheet

The first section of this sheet covers customer information. We request your company/institution that you are associated with. We then ask for information regarding the project this dataset will be associated with. This provides us the ability to provide analysis and insights from across individual datasets within a project. Finally, we request your preferred alias for this pool as it allows us to communicate with you regarding your order’s progress.
PropertyInstructions
Company/InstitutionFill in the company, institution, or lab for which this order is placed
Project name (optional)Fill in a project name
Order Alias (optional)Fill in your preferred alias for the order
This section provides us information on where to send the DNA pools once synthesized as well as the plate for return of the samples and sequencing. We will also use the shipping contact to inform the customer on any order updates.
Shipping address only needs to be filled in if you intend to run your own assay. Otherwise, if you are ordering end-to-end data generation then Instance will take care of running the assay and you will only receive the dataset.
PropertyInstructions
Shipping contactContact for shipping DNA pool to
Shipping email addressEmail address
Shipping address (full)Full address of the entity we are shipping the DNA to
Shipping phone numberPhone number of the entity we are shipping DNA to
Fill in billing information here
PropertyInstructions
Billing contactBilling contact
Billing email addressBilling email address
Billing address (full)Billing address
Billing phone numberBilling phone number
A common practice for our customers is providing amino acid sequences of proteins they want to test. We aim to provide the best DNA product possible and therefore request if amino acid sequences are provided the customer also provides the organism that will be used for the assay. This allows us to provide a codon optimized DNA sequence of that protein sequence.
PropertyInstructions
(optional) Host for codon optimizationIf submitting AA sequences, we will convert to DNA for the creation of the pool. Please inform us fo the organism for the assay and we will codon optimize the DNA sequence.
Testing your protein sequences is a core component of improving your model’s performance. With Instance Datasets, we offer two options. At Instance we have a set of assay partners that allow us to rapidly test your sequences. Alternatively, you can opt to run the assay yourself. In this section you can specify if you wish to run the assay or have us conduct it for you. If you are interested in running the assay, we ask what the yield is of plasmid/linear DNA for your assay.
PropertyInstructions
Assay conductorThe assay conductor is who will be performing the assay to test sequences. Can be either the customer or Instance
Requested yield (µg)If conducting your own assay. Please inform us how much plasmid/linear DNA you will need for your assay
2

Fill out Sequence order sheet

The sequence order sheet is split into five regions specified in separate columns. Sequence names can be placed in the first column. A final vector column is provided which allows you to specify the vector backbone to clone your DNA pool into. If the vector field is left blank, linear double stranded DNA will be provided.Blank Sequence order form

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Sequence order sheet structure

Each region can be left empty or can be either of these 3 options:
  1. Variable region must have exactly 1 variable region
  2. Barcode region must have exactly 1 barcode region
    • CMI/UMI barcodes that Instance will add for richer data insights
  3. Constant region (optional) up to 3 constant regions
    • eg. Promoters or Transcript termination signals
Let’s look at an example of a filled in form for ordering sequences:Sequence order form filled inIn the figure, you can see regions 1-4 filled in. Importantly, you can’t skip a region and go to the next one. As an example, I could not have filled in regions 1-3 and then gone to 5 without filling in 4.For this example, the regions correspond to:
  1. A constant sequence: CMV promoter.
  2. Variable sequence: The DNA molecules you wish to test for ability.
  3. A constant sequence: Transcript termination signal
  4. CMI/UMI barcode
Notice how the constant regions and CMI/UMI barcode aren’t populated across all rows. In this instance, all rows after the first, where these regions are filled in, will be assumed to have the same sequence as the first row.The regions will be concatenated together from 5 prime to 3 prime as shown in the above figure.
Depending on your assay, you may need your DNA pool cloned into a vector. We can manage this within our synthesis process. If you want to have your DNA pool cloned into a vector, simply provide the sequence of the vector backbone in the vector column. If you are using the same vector backbone across all sequences, only provide it in the first row. If no vector sequence is provided, the DNA pool will be shipped as linear double stranded DNA instead of plasmid.In the figure below we show how a DNA pool is cloned into your provided vector sequence. As you can see, the 5 prime portion of the vector sequence connects to the 5 prime portion of the DNA pool fragment. The 3 prime portion of the vector sequence connects to the 3 prime portion of the DNA pool fragment.Instance DNA fragment being cloned into a vector
Once we have received your sequences, we provide back a GenBank file to confirm the structure is as you intended.
There are a couple of requirements to keep in mind while designing your DNA pool:
  • Each region must be between 60-1000bp
  • All regions together (1-5, excluding vector) must be no larger than 2000bp
  • There must be a CMI/UMI barcode region specified
  • There must be a variable region containing DNA (or protein) sequences you wish to test