How to fill out an order form

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Overview of the order form

1. The Order information tab (1) requests general information about your order.
2. The DNA pool tab (2) is where you can input the pool sequences you want to test and gain data on.
3. The Construct and Assay info tab (3) collects information on your assay format, vector information (if necessary) and how many samples you would like to return for data generation.

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Step-by-step guide on how to fill out the order form

1

Fill out order information sheet

Customer information

The first section of this sheet covers customer information. We request your company/institution that you are associated with. We then ask for information regarding the project this dataset will be associated with. This provides us the ability to provide analysis and insights from across individual datasets within a project. Finally, we request your preferred alias for this pool as it allows us to communicate with you regarding your order’s progress.

Property	Instructions
Company/Institution	Fill in the company, institution, or lab for which this order is placed
Project name (optional)	Fill in a project name
Order Alias (optional)	Fill in your preferred alias for the order

Shipping information

This section provides us information on where to send the DNA pools once synthesized as well as the plate for return of the samples and sequencing. We will also use the shipping contact to inform the customer on any order updates.

Shipping information only needs to be provided if you are running your own assay. If you are ordering end-to-end data generation you do not need to complete this section. 

Property	Instructions
Shipping contact	Contact for shipping DNA pool to
Shipping email address	Email address
Shipping address (full)	Full address of the entity we are shipping the DNA to
Shipping phone number	Phone number of the entity we are shipping DNA to

Billing information

Please fill in where you would like billing and invoicing statements to be delivered to. 

Property	Instructions
Billing contact	Billing contact
Billing email address	Billing email address
Billing address (full)	Billing address
Billing phone number	Billing phone number

2

Provide your DNA pool details

This is where you enter the pool sequences you would like to test. They can be entered as amino acid sequences and these will be codon optimized, based on the host species you select. We also accept nucleotide sequences. We currently do not accept degenerate bases within the pool region. The maximum size accepted for each fragment is 1000 basepairs. 

Once you are done, please proceed to Tab 3: Construct and Assay Info. 

3

Complete the Construct and Assay Info

Tab 3 contains the critical information for your specific assay. There are four steps to complete:

Linear or Vector?

First, select if you would like your DNA pool provided as linear double-stranded (ds) DNA or as a cloned plasmid. If you would like the pool cloned into a plasmid, please specify the vector sequence. The pool will be cloned in between the last and first position of the vector sequence. The vector sequence should encompass the entirety of your construct, including promoters, stop codon etc. 

Flanking Sequences

If you have sequences that remain constant around the DNA pool sequence, you can put them here. This can be utilized with either dsDNA delivery or as a vector. If you are using a vector backbone, please paste 20 bp of the vector sequence upstream (5’) and downstream (3’) so we can confirm the location of the pool. 

Assay

If you are running your own assay, please provide some context on the assay you are using this material for. Please specify how much material (in ug) you require. If you are utilizing end to end data generation, this section is not needed.

Assay Return

If you are running your own assay, please enumerate how many samples do you expect to generate. These will be returned to Instance for analysis and data generation. If you are utilizing end to end data generation, this section is not needed

4

Submit!

Please submit the order form via email to orders@instance.bio. Please ensure all three tabs have been completed.