Summarized binding scores for each observed sequence variant and antigen combination. During the assay, translated candidate libraries are incubated with target antigens across a range of concentrations (typically 0 nM, 5 nM, 50 nM, and 500 nM). Binding scores quantify the relative enrichment of bound molecules compared to baseline abundance. Individual replicate measurements are averaged together, with each tested concentration pivoted into a dedicated score and standard error column.Grain: one row per (candidate_library_id, sino_catalog_id, variant_id).
Identifies this candidate across the dataset. Two candidates with identical sequences share the same candidate_id.
candidate_library_id
string
Identifies the pooled candidate library that was expressed and tested.
candidate_name
string
The customer-provided name for this candidate.
candidate_sequence
string
The customer-provided sequence for this candidate.
variant_id
string
Identifies the specific sequence variant observed for this candidate. Equals candidate_id when the observed sequence matches the candidate sequence exactly.
variant_sequence
string
The observed sequence variant after sequencing.
sino_catalog_id
string
Identifies the antigen using its Sino Biological catalog number.
antigen_gene_name
string
Gene name of the antigen.
antigen_sequence
string
Amino acid sequence of the antigen.
is_expressed
boolean
Indicates whether this candidate was expressed in at least one sample.
targeting
boolean
Indicates whether this candidate was designed to bind this antigen.
For each antigen concentration tested, the table includes a binding_score_{conc}nM column and a matching binding_score_{conc}nM_std column. The standard workflow uses 5, 50, and 500 nM.
Column
Type
Description
binding_score_5nM
float
Mean binding score at 5 nM, averaged across replicates.
binding_score_5nM_std
float
Standard error of the mean binding score at 5 nM across replicates.
binding_score_50nM
float
Mean binding score at 50 nM, averaged across replicates.
binding_score_50nM_std
float
Standard error of the mean binding score at 50 nM across replicates.
binding_score_500nM
float
Mean binding score at 500 nM, averaged across replicates.
binding_score_500nM_std
float
Standard error of the mean binding score at 500 nM across replicates.
File:replicate-binding-scores-YYYYMMDD.parquetContains the individual replicate binding measurements that are averaged together to produce the summarized binding_scores table. Each row captures one candidate’s binding score at a single antigen concentration in a single binding sample, linked to its corresponding expression sample, along with the underlying Unique Molecular Identifier (UMI) counts that support the score. Use this table to inspect replicate-to-replicate variability and assess assay reproducibility.Grain: one row per (expression_sample_id, binding_sample_id, variant_id).
Column
Type
Description
candidate_id
string
Identifies this candidate across the dataset. Two candidates with identical sequences share the same candidate_id.
candidate_library_id
string
Identifies the pooled candidate library that was expressed and tested.
candidate_name
string
The customer-provided name for this candidate.
candidate_sequence
string
The customer-provided sequence for this candidate.
variant_id
string
Identifies the specific sequence variant observed for this candidate. Equals candidate_id when the observed sequence matches the candidate sequence exactly.
variant_sequence
string
The observed sequence variant after sequencing.
binding_sample_id
string
Identifies the binding sample this measurement is based on, matching in dim_samples.
binding_score
float
Binding score for this candidate in this sample at this concentration. Empty when Unique Molecular Identifier (UMI) counts do not meet quality thresholds.
binding_umi_count
integer
Number of distinct molecules (UMIs) observed for this candidate in the binding sample.
expression_sample_id
string
Identifies the expression sample this measurement is based on, matching in dim_samples.
expression_umi_count
integer
Number of distinct molecules (UMIs) observed for this candidate in the expression sample.
sino_catalog_id
string
Identifies the antigen using its Sino Biological catalog number.
antigen_concentration_nM
float
Concentration of the antigen used for this measurement, in nanomolar (nM).
antigen_gene_name
string
Gene name of the antigen.
antigen_sequence
string
Amino acid sequence of the antigen.
is_expressed
boolean
Indicates whether this candidate was expressed in this sample.
targeting
boolean
Indicates whether this candidate was designed to bind this antigen.
